This increases enzyme activity and more product will be formed. In tobacco leaf cells peroxisomes, a paracrystalline core can take up most of the volume. For example, if there was still 1cm 3 of water remaining in the conical flask and beaker combined, then an 80% concentration of hydrogen peroxide would be closer to 79%. Why do you think this is so? Place 40 ml of H 2O 2 into a new beaker. I did five different concentrations and repeated the experiment three times to compare the results and make sure it is accurate then I took the average result to work from onto my graph.
In this lab, you will study the catalase found in liver cells. I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks. The disk floats will not sink. It seems more likely to me that some assumption made in calculating Catalase's efficiency relative to the diffusion limit is not valid. This means there will be twice as many successful collisions. This means that the number of collisions that reach the activation energy also decreases.
Since our bodies have enzymes though hundreds of chemical reactions a day. Enzymes force reactions, in cells, to maintain a speed necessary for life. To test for the presence of monosaccharides and reducing disaccharide sugars in food, the food sample is dissolved in water, and a small amount of Benedict's reagent is added. Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time. Evaluation: Even though I get the results I was hoping for I still got some anomalous results in my graph.
By preventing excessive H 2O 2 build up Catalase allows important cellular processes which produce H 2O 2 as a byproduct to take place safely. Students will determine how enzyme concentration affects reaction rate. To work out this experiment, I am going to use a formula of: 2H2O2-using catalase to become 2H2O+O2 I will use a set of five pH's to get my readings from the collected oxygen. How does the blended liver look after heating? Can you explain the difference? Each enzyme just performs one particular reaction so they are all very specific. I predicted hat the liver would have a faster reaction rate than the potato when mixed with the hydrogen peroxide. I also made sure that I used the same mass of potato and liver, as I wanted to compare them. Most enzymes only function under optimal environmental conditions.
The following graph shows reaction rates of various enzymes in the body. You might have noticed that when doing the extra steps in the procedure. The purpose of this experiment was find the different factors that can affect Catalase-Peroxide reactions. Do this first to ensure that the mixtures have enough time to reach a constant temperature instead of putting them in for a short time. This shows that the liver enzyme catalase is working to start the chemical reaction that breaks down the hydrogen peroxide that would be harmful to the body into less dangerous compounds. This could work in vivo but not in vitro in a homogeneous system for it needs structure for this to work.
At very high temperatures, enzymes, because they are made of protein, can be denatured or destroyed. All enzymes have an active site. Enzymes are designed to work most effectively at a specific temperature and pH. In our body we produce Hydrogen Peroxide. Overall, I believe my experiment went well and that I gained sufficient results because I repeated each concentration three times and investigated eight concentrations in total.
By doing this, it will ensure that the test is as fair as I can make it. All enzyme catalysed reactions are reversible. These cups will be used to dip your filter paper disks. Within your liver, as within every tissue in the body, many chemical reactions occur. Once you add the enzyme, does the catalase react with the hydrogen peroxide? During the experiment, factors such as: amount of enzyme, substrate concentration, and pH were tested to find a correlation with enzyme activity.
This can be explained by the Maxwell-Boltzmann distribution curve. To help crush the liver a pinch of sand was placed in the mortar. Place the other set in a warm water bath not boiling. I don't know whether this effect can rightly be called 'catalysis', or whether it is significant in measurements of rates of Catalase catlysed decomposition of H 2O 2. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added.
Ask an Expert The Ask an Expert Forum is intended to be a place where students can go to find answers to science questions that they have been unable to find using other resources. Though in theory, this should be the trend, my results did not demonstrate this pattern. The performance of catalase is dependent on many factors, so the purpose of the lab was to find the ideal concentration and temperature for catalase. Therefore it is true to say that in theory, rate µ concentration. This could have affected my results for several reasons. Catalase also uses Hydrogen Peroxide to oxidise toxins including Phenols, Formic Acid, Formaldehyde and Alcohols. The job of catalase in this experiment was to accelerate the breakdown of hydrogen peroxide into water and oxygen gas.